Hotspot

ndhJ

Genome · 54396-54914 · 519 bp

Back to Sarmentypnum

Pi 0.00193

MarkerSeek score 48.30

Primer available yes

Label ndhJ

Feature Metadata

label_name: ndhJ
feature_type: gene
region: Genome
length_bp: 519
variable_sites: 1
pi: 0.00193
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ_p1	AAGCCGGAATAGCTCAGTTG	AGCAAATGCCTGAGCCTAAA	50.0	45.0	58.0	57.8	1234 bp	1.0	78.1
ndhJ_p2	AAGCCGGAATAGCTCAGTTG	GGTTGTCCACCAAAACCAGA	50.0	50.0	58.0	58.2	1103 bp	1.0	78.1
ndhJ_p3	AAGCCGGAATAGCTCAGTTG	GAGCAAATGCCTGAGCCTAA	50.0	50.0	58.0	58.2	1235 bp	1.0	77.9
ndhJ_p4	TGCAGAGACTCAATGGAAGC	AGCAAATGCCTGAGCCTAAA	50.0	45.0	57.9	57.8	1485 bp	1.0	77.5
ndhJ_p5	GGGTATGGCGGAATTGGTAG	AGCAAATGCCTGAGCCTAAA	55.0	45.0	58.1	57.8	1641 bp	1.0	77.5