Hotspot

ndhI

Genome · 103495-104046 · 552 bp

Back to Sarmentypnum

Pi 0.00181

MarkerSeek score 57.10

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: Genome
length_bp: 552
variable_sites: 1
pi: 0.00181
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	TCCTTTTTGTTTCTATTGTTCTGA	ACAGCTTCTTTTCAAGTTCT	29.2	35.0	54.9	52.9	755-756 bp	1.0	40.1
ndhI_p2	TCCTTTTTGTTTCTATTGTTCTGA	AACAGCTTCTTTTCAAGTTCT	29.2	33.3	54.9	53.9	756-757 bp	1.0	40.1
ndhI_p3	TCCTTTTTGTTTCTATTGTTCTGA	ACAGCTTCTTTTCAAGTTCTT	29.2	33.3	54.9	53.9	755-756 bp	1.0	40.1
ndhI_p4	TCCTTTTTGTTTCTATTGTTCTGA	AACAGCTTCTTTTCAAGTTCTT	29.2	31.8	54.9	54.7	756-757 bp	1.0	40.1
ndhI_p5	TCCTTTTTGTTTCTATTGTTCTGA	ACAGCTTCTTTTCAAGTTCTTT	29.2	31.8	54.9	54.7	755-756 bp	1.0	40.1