Hotspot

chlB

Genome · 25975-27507 · 1533 bp

Back to Sarmentypnum

Pi 0

MarkerSeek score 27.90

Primer available yes

Label chlB

Feature Metadata

label_name: chlB
feature_type: gene
region: Genome
length_bp: 1533
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 27.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlB_p1	GAGGTTCGAATCCTTCCGTC	TGGGATTCATAAACTTTTCCTACA	55.0	33.3	58.1	56.0	2019 bp	1.0	40.2
chlB_p2	ATCTATTTATGGGGCGTCGC	TGGGATTCATAAACTTTTCCTACA	50.0	33.3	57.9	56.0	2076 bp	1.0	40.2
chlB_p3	GAGGTTCGAATCCTTCCGTC	AATTCTTTTTGTCAATGCTGT	55.0	28.6	58.1	52.8	2329 bp	1.0	40.2
chlB_p4	ATCTATTTATGGGGCGTCGC	AATTCTTTTTGTCAATGCTGT	50.0	28.6	57.9	52.8	2386 bp	1.0	40.2
chlB_p5	GGTAAGGCAGCAGGTTTTGA	TGGGATTCATAAACTTTTCCTACA	50.0	33.3	58.4	56.0	2051 bp	1.0	40.2