Hotspot

atpI

Genome · 18048-18794 · 747 bp

Back to Sarmentypnum

Pi 0

MarkerSeek score 27.00

Primer available yes

Label atpI

Feature Metadata

label_name: atpI
feature_type: gene
region: Genome
length_bp: 747
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 27.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI_p1	GCAAATGATGATGCTCGAGC	TACCTTGACCAATCCCAGGT	50.0	50.0	58.0	57.9	1582 bp	1.0	79.5
atpI_p2	AAATCAAAAGTGGCTTGGCG	TACCTTGACCAATCCCAGGT	45.0	50.0	57.8	57.9	1910 bp	1.0	78.5
atpI_p3	GCAAATGATGATGCTCGAGC	GCAGCAGTACCTTGACCAAT	50.0	50.0	58.0	58.2	1589 bp	1.0	78.5
atpI_p4	TTGCAGGTCGTTGTAATCCC	TACCTTGACCAATCCCAGGT	50.0	50.0	58.2	57.9	1521 bp	1.0	78.3
atpI_p5	GCAAATGATGATGCTCGAGC	GCAGTACCTTGACCAATCCC	50.0	55.0	58.0	58.2	1586 bp	1.0	77.9