Hotspot

atpA

Genome · 21081-22604 · 1524 bp

Back to Sarmentypnum

Pi 0.000656

MarkerSeek score 46.60

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: Genome
length_bp: 1524
variable_sites: 1
pi: 0.000656
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	AGCGAGAAAGCCGAATGAAT	ACCCTTAGCCTTCCAAGCTA	45.0	50.0	58.0	58.0	2443-2452 bp	1.0	79.6
atpA_p2	AGCGAGAAAGCCGAATGAAT	CCCACTACCCGCCTTTATTC	45.0	55.0	58.0	58.0	2406-2415 bp	1.0	79.6
atpA_p3	AGCGAGAAAGCCGAATGAAT	CGCCGACTATAACCCTTAGC	45.0	55.0	58.0	57.9	2454-2463 bp	1.0	79.4
atpA_p4	AGCGAGAAAGCCGAATGAAT	GCGCCGACTATAACCCTTAG	45.0	55.0	58.0	57.9	2455-2464 bp	1.0	79.4
atpA_p5	GGGGCAGGCTTAGTTTTTCT	ACCCTTAGCCTTCCAAGCTA	50.0	50.0	58.1	58.0	2468-2477 bp	1.0	79.2