Hotspot

petA-psbJ

Genome · 66413-67307 · 895 bp

Back to Sarmentypnum

Pi 0.00112

MarkerSeek score 59.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 895
variable_sites: 1
pi: 0.00112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGCATCCGTTATATTAGCACA	GGCTCATACTCTGGATTAGGC	38.1	52.4	55.2	57.7	1013-1025 bp	1.0	40.1
petA-psbJ_p2	TGCATCCGTTATATTAGCACA	ACTCTGGATTAGGCTCATCGT	38.1	47.6	55.2	58.3	1006-1018 bp	1.0	40.1
petA-psbJ_p3	TGCATCCGTTATATTAGCACA	ACTCTGGATTAGGCTCATCG	38.1	50.0	55.2	56.5	1006-1018 bp	1.0	40.1
petA-psbJ_p4	TGCATCCGTTATATTAGCACA	CTCTGGATTAGGCTCATCGT	38.1	50.0	55.2	56.5	1005-1017 bp	1.0	40.1
petA-psbJ_p5	TGCATCCGTTATATTAGCACAA	GGCTCATACTCTGGATTAGGC	36.4	52.4	56.0	57.7	1013-1025 bp	1.0	40.1