Hotspot

trnT-GGU-psbD

Genome · 40583-41233 · 651 bp

Back to Sarmentypnum

Pi 0.00154

MarkerSeek score 59.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: Genome
length_bp: 651
variable_sites: 1
pi: 0.00154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	AAAACGGTCTCTTCGTAGCC	55.0	50.0	58.1	57.9	793 bp	1.0	79.0
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	AAAACGGTCTCTTCGTAGCC	50.0	50.0	58.1	57.9	796 bp	1.0	78.8
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	TCTTCGTAGCCAGTCATCCA	55.0	50.0	58.1	58.2	784 bp	1.0	78.4
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	TCTTCGTAGCCAGTCATCCA	50.0	50.0	58.1	58.2	787 bp	1.0	78.2
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	AAAACGGTCTCTTCGTAGCC	50.0	50.0	58.3	57.9	770 bp	1.0	77.2