Hotspot

ycf35

Genome · 88448-88852 · 405 bp

Back to Sargassum

Pi 0.0339

MarkerSeek score 59.20

Primer available yes

Label ycf35

Feature Metadata

label_name: ycf35
feature_type: gene
region: Genome
length_bp: 405
variable_sites: 41
pi: 0.0339
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf35_p1	TCCACCTTTTTAATGCCCCC	TAAAGTCAAGGGTGGTTGGC	50.0	50.0	58.1	58.0	1180-1213 bp	1.0	91.5
ycf35_p2	TCCACCTTTTTAATGCCCCC	GAGGGTCACTGGTTCGAATC	50.0	55.0	58.1	58.0	1131-1164 bp	1.0	91.5
ycf35_p3	AGGTGGAAGCGTTTCAGTTT	GAGGGTCACTGGTTCGAATC	45.0	55.0	57.9	58.0	1037-1059 bp	0.615385	76.1
ycf35_p4	AGGTGGAAGCGTTTCAGTTT	TAAAGTCAAGGGTGGTTGGC	45.0	50.0	57.9	58.0	1086-1108 bp	0.615385	75.8
ycf35_p5	TGCCCCCATTCGAAATTTGA	TAAAGTCAAGGGTGGTTGGC	45.0	50.0	58.1	58.0	1167-1200 bp	0.538462	70.8