Hotspot

ycf33-ycf39

Genome · 84344-84611 · 268 bp

Back to Sargassum

Pi 0.0459

MarkerSeek score 73.10

Primer available yes

Label ycf33-ycf39

Feature Metadata

label_name: ycf33-ycf39
feature_type: igs
region: Genome
length_bp: 268
variable_sites: 36
pi: 0.0459
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf33-ycf39_p1	AAAAACCTTGGATAACGTAATAA	CCTGTTCCTCCTAAAATAAGT	26.1	38.1	52.4	52.5	334-353 bp	1.0	52.0
ycf33-ycf39_p2	AAAACCTTGGATAACGTAATAAA	CCTGTTCCTCCTAAAATAAGT	26.1	38.1	52.4	52.5	333-352 bp	1.0	52.0
ycf33-ycf39_p3	AAAAACCTTGGATAACGTAATAAA	CCTGTTCCTCCTAAAATAAGT	25.0	38.1	53.2	52.5	334-353 bp	1.0	52.0
ycf33-ycf39_p4	AAACCTTGGATAACGTAATAAAT	CCTGTTCCTCCTAAAATAAGT	26.1	38.1	52.2	52.5	332-351 bp	1.0	52.0
ycf33-ycf39_p5	AACCTTGGATAACGTAATAAATT	CCTGTTCCTCCTAAAATAAGT	26.1	38.1	52.2	52.5	331-350 bp	1.0	52.0