Hotspot

rrn5-ycf19

Genome · 78812-79370 · 559 bp

Back to Sargassum

Pi 0.049

MarkerSeek score 81.40

Primer available yes

Label rrn5-ycf19

Feature Metadata

label_name: rrn5-ycf19
feature_type: igs
region: Genome
length_bp: 559
variable_sites: 82
pi: 0.049
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-ycf19_p1	CGAACTCAATGGTGAAACGC	ATCTTCCAGTTTCCAAAGCT	50.0	40.0	58.0	54.8	677-701 bp	0.923077	61.2
rrn5-ycf19_p2	CGAACTCAATGGTGAAACGC	AATCTTCCAGTTTCCAAAGCT	50.0	38.1	58.0	55.7	678-702 bp	0.923077	59.9
rrn5-ycf19_p3	CGAACTCAATGGTGAAACGC	TCTTCCAGTTTCCAAAGCTA	50.0	40.0	58.0	54.2	676-700 bp	0.923077	56.0
rrn5-ycf19_p4	CGAACTCAATGGTGAAACGC	AATCTTCCAGTTTCCAAAGC	50.0	40.0	58.0	54.1	678-702 bp	0.923077	55.1
rrn5-ycf19_p5	CGAACTCAATGGTGAAACGC	TCTTCCAGTTTCCAAAGCTAA	50.0	38.1	58.0	55.0	676-700 bp	0.923077	55.0