Hotspot

ccsA-trnL-UAG

Genome · 67164-67376 · 213 bp

Back to Sargassum

Pi 0.0873

MarkerSeek score 75.60

Primer available yes

Label ccsA-trnL-UAG

Feature Metadata

label_name: ccsA-trnL-UAG
feature_type: igs
region: Genome
length_bp: 213
variable_sites: 52
pi: 0.0873
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-trnL-UAG_p1	GCTTTTGCTGAATCTGCTCG	AATGGCTAATCGGGCTTTGT	50.0	45.0	58.1	57.9	772-894 bp	1.0	90.0
ccsA-trnL-UAG_p2	CCTTGCGAAAGGAGTAGCTT	AATGGCTAATCGGGCTTTGT	50.0	45.0	57.9	57.9	977-995 bp	0.384615	61.8
ccsA-trnL-UAG_p3	CCTTGCGAAAGGAGTAGCTT	TTTACCCGTATTAGCCGCAG	50.0	50.0	57.9	57.8	1335-1357 bp	0.307692	57.1
ccsA-trnL-UAG_p4	CGCGTTAGTACCTGCTCTAC	AATGGCTAATCGGGCTTTGT	55.0	45.0	57.9	57.9	1172-1183 bp	0.307692	55.1
ccsA-trnL-UAG_p5	CGCGTTAGTACCTGCTCTAC	TTTACCCGTATTAGCCGCAG	55.0	50.0	57.9	57.8	1530-1541 bp	0.230769	50.8