Hotspot

rbcS-trnE-UUC

Genome · 45155-45447 · 293 bp

Back to Sargassum

Pi 0.0562

MarkerSeek score 73.10

Primer available yes

Label rbcS-trnE-UUC

Feature Metadata

label_name: rbcS-trnE-UUC
feature_type: igs
region: Genome
length_bp: 293
variable_sites: 49
pi: 0.0562
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcS-trnE-UUC_p1	TATTGTACAACGTCCTGCCG	GTTATACCTTCAGCACGCCA	50.0	50.0	58.0	58.0	1143-1187 bp	0.923077	86.3
rbcS-trnE-UUC_p2	TATTGTACAACGTCCTGCCG	TGTTATACCTTCAGCACGCC	50.0	50.0	58.0	58.0	1144-1188 bp	0.846154	82.7
rbcS-trnE-UUC_p3	ACCTGCAGGCGATAGATACT	GTTATACCTTCAGCACGCCA	50.0	50.0	58.0	58.0	1038-1069 bp	0.461538	62.9
rbcS-trnE-UUC_p4	ACCTGCAGGCGATAGATACT	TGTTATACCTTCAGCACGCC	50.0	50.0	58.0	58.0	1039-1070 bp	0.461538	62.9
rbcS-trnE-UUC_p5	TTACGTACCGCAGCAAGTAC	GTTATACCTTCAGCACGCCA	50.0	50.0	58.0	58.0	1733-1734 bp	0.307692	57.2