Hotspot

psaD-trnS-UGA

Genome · 22899-23071 · 173 bp

Back to Sargassum

Pi 0.0755

MarkerSeek score 75.10

Primer available yes

Label psaD-trnS-UGA

Feature Metadata

label_name: psaD-trnS-UGA
feature_type: igs
region: Genome
length_bp: 173
variable_sites: 40
pi: 0.0755
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaD-trnS-UGA_p1	AAAATCCTAACCCAGCTTCT	AAATCAGACGCAATCGACCA	40.0	45.0	54.2	57.9	227-255 bp	0.923077	57.7
psaD-trnS-UGA_p2	AAAATCCTAACCCAGCTTCT	ATCAGACGCAATCGACCATC	40.0	50.0	54.2	58.1	225-253 bp	0.923077	57.3
psaD-trnS-UGA_p3	AAAAATCCTAACCCAGCTTCT	AAATCAGACGCAATCGACCA	38.1	45.0	55.1	57.9	228-256 bp	0.923077	56.7
psaD-trnS-UGA_p4	AAAATCCTAACCCAGCTTCT	CAATCGACCATCTCTGCCAT	40.0	50.0	54.2	57.7	217-245 bp	0.923077	56.4
psaD-trnS-UGA_p5	AAAAATCCTAACCCAGCTTCT	ATCAGACGCAATCGACCATC	38.1	50.0	55.1	58.1	226-254 bp	0.923077	56.3