Hotspot

trnD-GUC-trnM-CAU

Genome · 9436-9612 · 177 bp

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Pi 0.0503

MarkerSeek score 76.30

Primer available yes

Label trnD-GUC-trnM-CAU

Feature Metadata

label_name: trnD-GUC-trnM-CAU
feature_type: igs
region: Genome
length_bp: 177
variable_sites: 28
pi: 0.0503
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnM-CAU_p1	CCGTGACAGGGTGATACTCT	CGACCAAGGGCTTATGAGTC	55.0	55.0	58.5	58.1	262-270 bp	1.0	87.0
trnD-GUC-trnM-CAU_p2	TAACTTTCGGGATTGACGGG	CGACCAAGGGCTTATGAGTC	50.0	55.0	57.6	58.1	302-310 bp	1.0	86.9
trnD-GUC-trnM-CAU_p3	TAACTTTCGGGATTGACGGG	GGGCTTATGAGTCCCCTACT	50.0	55.0	57.6	57.9	295-303 bp	1.0	86.8
trnD-GUC-trnM-CAU_p4	CCGTGACAGGGTGATACTCT	GGGCTTATGAGTCCCCTACT	55.0	55.0	58.5	57.9	255-263 bp	1.0	86.7
trnD-GUC-trnM-CAU_p5	TAACTTTCGGGATTGACGGG	GACTCTGGCTGGACTTGAAC	50.0	55.0	57.6	58.2	325-333 bp	1.0	85.1