Hotspot

ycf47-trnM-CAU

Genome · 4117-4719 · 603 bp

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Pi 0.0431

MarkerSeek score 73.40

Primer available yes

Label ycf47-trnM-CAU

Feature Metadata

label_name: ycf47-trnM-CAU
feature_type: igs
region: Genome
length_bp: 603
variable_sites: 85
pi: 0.0431
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf47-trnM-CAU_p1	GTTTGGGGTCGTAACGGATT	AAATTGCATCTGGCTGGGTT	50.0	45.0	58.2	58.4	1156-1181 bp	1.0	86.2
ycf47-trnM-CAU_p2	GTTTGGGGTCGTAACGGATT	ATGAGTCCGTTGCCTTCAAC	50.0	50.0	58.2	58.5	1103-1128 bp	1.0	85.6
ycf47-trnM-CAU_p3	GTTTGGGGTCGTAACGGATT	TCGAACCAGCGTTGTTCTAA	50.0	45.0	58.2	57.5	1137-1162 bp	1.0	85.1
ycf47-trnM-CAU_p4	GTTTGGGGTCGTAACGGATT	TCGTACTTCCTGCACCTTTC	50.0	50.0	58.2	57.5	1799-1832 bp	1.0	84.5
ycf47-trnM-CAU_p5	GTTTGGGGTCGTAACGGATT	CGTACTTCCTGCACCTTTCT	50.0	50.0	58.2	57.5	1798-1831 bp	1.0	84.5