Hotspot

chlI-trnF-GAA

Genome · 1897-2091 · 195 bp

Back to Sargassum

Pi 0.0777

MarkerSeek score 84.80

Primer available yes

Label chlI-trnF-GAA

Feature Metadata

label_name: chlI-trnF-GAA
feature_type: igs
region: Genome
length_bp: 195
variable_sites: 50
pi: 0.0777
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlI-trnF-GAA_p1	CGCTTACGAAAAGACCCTTT	GAACTGGTGACACGAGGATC	45.0	55.0	56.4	58.0	335-427 bp	1.0	79.0
chlI-trnF-GAA_p2	CGCTTACGAAAAGACCCTTT	TCCACTGCTCTACCAACTGA	45.0	50.0	56.4	58.0	310-402 bp	1.0	78.9
chlI-trnF-GAA_p3	CGCTTACGAAAAGACCCTTT	TTTGAACTGGTGACACGAGG	45.0	50.0	56.4	58.1	338-430 bp	1.0	78.6
chlI-trnF-GAA_p4	TGTTTACGTCATCGCTTACG	GAACTGGTGACACGAGGATC	45.0	55.0	56.3	58.0	347-439 bp	0.923077	75.5
chlI-trnF-GAA_p5	TGTTTACGTCATCGCTTACG	TCCACTGCTCTACCAACTGA	45.0	50.0	56.3	58.0	322-414 bp	0.923077	75.4