Hotspot

ndhD-psaC

SSC · 116218-116358 · 141 bp

Back to Sarcopyramis

Pi 0.0429

MarkerSeek score 63.70

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 141
variable_sites: 6
pi: 0.0429
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	ACGTTTCTGTCCTCCCCATA	CCTTGGGATGGATGTAAGGC	50.0	55.0	58.1	58.0	779-780 bp	1.0	80.6
ndhD-psaC_p2	ACGTTTCTGTCCTCCCCATA	CAAATTGCTTCTGCTCCACG	50.0	50.0	58.1	58.0	755-756 bp	1.0	80.5
ndhD-psaC_p3	ACGTTTCTGTCCTCCCCATA	GGCATGAAACAACTCGAAGC	50.0	50.0	58.1	58.0	658-659 bp	1.0	80.5
ndhD-psaC_p4	ACGTTTCTGTCCTCCCCATA	AAACAACTCGAAGCATGGGT	50.0	45.0	58.1	58.0	653 bp	0.5	59.4
ndhD-psaC_p5	ACGTTTCTGTCCTCCCCATA	ATCTTAGCCAACCCAGGAGT	50.0	50.0	58.1	58.0	1016 bp	0.5	59.4