Hotspot

trnR-ACG-trnN-GUU

IRb · 107564-108158 · 595 bp

Back to Sarcopyramis

Pi 0.00349

MarkerSeek score 64.20

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 595
variable_sites: 2
pi: 0.00349
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	714-736 bp	1.0	80.9
trnR-ACG-trnN-GUU_p2	GGGTACTCCCGAGAACAGAT	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	58.2	58.0	763-785 bp	1.0	80.7
trnR-ACG-trnN-GUU_p3	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.7	58.0	751-773 bp	1.0	80.2
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	GCTGTTAACTGACTGGTCGT	55.0	50.0	57.8	57.8	678-700 bp	1.0	79.9
trnR-ACG-trnN-GUU_p5	GGGTACTCCCGAGAACAGAT	GCTGTTAACTGACTGGTCGT	55.0	50.0	58.2	57.8	727-749 bp	1.0	79.6