Hotspot

rpl33-rps18

LSC · 66955-67169 · 215 bp

Back to Sarcopyramis

Pi 0.014

MarkerSeek score 47.50

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 215
variable_sites: 3
pi: 0.014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CGAGTCGCTTGGAATTGAGA	AGAGTCGCTTGGATTTTTCCA	50.0	42.9	58.0	57.9	311 bp	1.0	71.6
rpl33-rps18_p2	CGAGTCGCTTGGAATTGAGA	CGAAAGAGTCGCTTGGATTT	50.0	45.0	58.0	56.5	315 bp	1.0	68.4
rpl33-rps18_p3	AATCGACACAATACGCCGAG	AGAGTCGCTTGGATTTTTCCA	50.0	42.9	58.4	57.9	327 bp	1.0	68.1
rpl33-rps18_p4	AGAATCGACACAATACGCCG	AGAGTCGCTTGGATTTTTCCA	50.0	42.9	58.4	57.9	329 bp	1.0	68.1
rpl33-rps18_p5	GAATCGACACAATACGCCGA	AGAGTCGCTTGGATTTTTCCA	50.0	42.9	58.4	57.9	328 bp	1.0	68.0