Hotspot

cemA-petA

LSC · 61547-61747 · 201 bp

Back to Sarcopyramis

Pi 0.0199

MarkerSeek score 63.90

Primer available yes

Label cemA-petA

Feature Metadata

label_name: cemA-petA
feature_type: igs
region: LSC
length_bp: 201
variable_sites: 4
pi: 0.0199
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA-petA_p1	TGGCCTTGTTTCTACTTTTCCA	ACGCATTTGAGATAGATGCTTG	40.9	40.9	58.1	57.2	437 bp	1.0	41.5
cemA-petA_p2	TGGCCTTGTTTCTACTTTTCCA	CGCATTTGAGATAGATGCTTGAG	40.9	43.5	58.1	58.0	436 bp	1.0	40.7
cemA-petA_p3	TGGCCTTGTTTCTACTTTTCCA	CGCATTTGAGATAGATGCTTGA	40.9	40.9	58.1	56.9	436 bp	1.0	40.7
cemA-petA_p4	TGGCCTTGTTTCTACTTTTCCA	ACGCATTTGAGATAGATGCT	40.9	40.0	58.1	54.9	437 bp	1.0	40.7
cemA-petA_p5	GGCCTTGTTTCTACTTTTCCAG	ACGCATTTGAGATAGATGCTTG	45.5	40.9	57.5	57.2	436 bp	1.0	40.7