Hotspot

trnM-CAU-atpE

LSC · 52560-52736 · 177 bp

Back to Sarcopyramis

Pi 0.0282

MarkerSeek score 68.70

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 177
variable_sites: 5
pi: 0.0282
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	GGCAGGAGTCATTGGTTCAA	GAAATCTAGCTGTCCGACGG	50.0	55.0	58.1	58.2	292-297 bp	1.0	79.4
trnM-CAU-atpE_p2	GGCAGGAGTCATTGGTTCAA	GGAAATCTAGCTGTCCGACG	50.0	55.0	58.1	58.2	293-298 bp	1.0	79.4
trnM-CAU-atpE_p3	GGCAGGAGTCATTGGTTCAA	AGGGAAATCTAGCTGTCCGA	50.0	50.0	58.1	57.8	295-300 bp	1.0	79.3
trnM-CAU-atpE_p4	GGCAGGAGTCATTGGTTCAA	GGGAAATCTAGCTGTCCGAC	50.0	55.0	58.1	57.8	294-299 bp	1.0	78.8
trnM-CAU-atpE_p5	TGAGTTCCTTTGCGTTTGGT	GAAATCTAGCTGTCCGACGG	45.0	55.0	58.2	58.2	370-375 bp	1.0	77.9