Hotspot

trnT-GGU-psbD

LSC · 31924-33084 · 1161 bp

Back to Sarcopyramis

Pi 0.013

MarkerSeek score 66.80

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1161
variable_sites: 15
pi: 0.013
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	AGGAGCCCCTTTAACTCAGT	GAAACGGTCCCTACGTAACC	50.0	55.0	58.0	58.0	1321-1331 bp	1.0	80.9
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTACGTAACC	55.0	55.0	58.1	58.0	1303-1313 bp	1.0	80.8
trnT-GGU-psbD_p3	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.1	58.0	1306-1316 bp	1.0	80.6
trnT-GGU-psbD_p4	GAGCCCCTTTAACTCAGTGG	GAAACGGTCCCTACGTAACC	55.0	55.0	57.9	58.0	1319-1329 bp	1.0	80.4
trnT-GGU-psbD_p5	GGAGCCCCTTTAACTCAGTG	GAAACGGTCCCTACGTAACC	55.0	55.0	57.9	58.0	1320-1330 bp	1.0	80.4