Hotspot

matK-trnK-UUU

LSC · 3423-4197 · 775 bp

Back to Sarcopyramis

Pi 0.00903
MarkerSeek score 63.40
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
775
variable_sites
7
pi
0.00903
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 AGGAAGTTGTGTTGCCAAGA AACGGTAGAGTACTCGGCTT 45.0 50.0 57.6 58.2 855 bp 1.0 75.8
matK-trnK-UUU_p2 AGGAAGTTGTGTTGCCAAGA ACGGTAGAGTACTCGGCTTT 45.0 50.0 57.6 58.2 854 bp 1.0 75.8
matK-trnK-UUU_p3 AGGAAGTTGTGTTGCCAAGA GGGTTGCTAACTCAACGGTA 45.0 50.0 57.6 57.5 868 bp 1.0 73.5
matK-trnK-UUU_p4 AGGAAGTTGTGTTGCCAAGA TTCGGGTTGCTAACTCAACG 45.0 50.0 57.6 58.5 871 bp 1.0 73.2
matK-trnK-UUU_p5 AGGAAGTTGTGTTGCCAAGAT ACGGTAGAGTACTCGGCTTT 42.9 50.0 58.1 58.2 854 bp 1.0 70.9