Hotspot

rps15

SSC · 118928-119191 · 264 bp

Back to Sarcandra

Pi 0.508

MarkerSeek score 75.80

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 264
variable_sites: 120
pi: 0.508
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	CCAGATCGAAATGACTGGCA	GAAAGGAGTCTGTGCACCAA	50.0	50.0	58.0	58.0	850 bp	0.5	59.5
rps15_p2	CCAGATCGAAATGACTGGCA	TTCCTCTCCGCCAATAATGC	50.0	50.0	58.0	58.0	1096 bp	0.5	59.5
rps15_p3	ACCAGATCGAAATGACTGGC	GAAAGGAGTCTGTGCACCAA	50.0	50.0	58.0	58.0	851 bp	0.5	59.5
rps15_p4	ACCAGATCGAAATGACTGGC	TTCCTCTCCGCCAATAATGC	50.0	50.0	58.0	58.0	1097 bp	0.5	59.5
rps15_p5	CCAGATCGAAATGACTGGCA	AACACCATGCATTGATGGGT	50.0	45.0	58.0	58.1	532 bp	0.5	59.3