Hotspot

psaC

SSC · 124971-125216 · 246 bp

Back to Sarcandra

Pi 0.469

MarkerSeek score 74.60

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 98
pi: 0.469
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	GCTATTGGGCCAGCTATTGT	GTAGTGATGAAGCCCGTCAG	50.0	55.0	58.0	58.1	993 bp	0.5	59.3
psaC_p2	GCTATTGGGCCAGCTATTGT	CCCCCTGCAGTGTACAAAAT	50.0	50.0	58.0	58.1	1221 bp	0.5	59.3
psaC_p3	GCTATTGGGCCAGCTATTGT	AACCGCGAATCTCGAGTAAC	50.0	50.0	58.0	58.1	1038 bp	0.5	59.1
psaC_p4	GCTATTGGGCCAGCTATTGT	CAACCGCGAATCTCGAGTAA	50.0	50.0	58.0	58.1	1039 bp	0.5	59.1
psaC_p5	CGAGATTGATGGGGAGTTGG	GTAGTGATGAAGCCCGTCAG	55.0	55.0	58.0	58.1	1238 bp	0.5	59.1