Hotspot

ndhI

SSC · 122734-123291 · 558 bp

Back to Sarcandra

Pi 0.456

MarkerSeek score 81.40

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 558
variable_sites: 230
pi: 0.456
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	CATCGAGGAGCCGTATGAAG	GACATTTATGGCTCCCACGT	55.0	50.0	57.9	58.0	1807 bp	0.5	59.2
ndhI_p2	GCGGGTGGAATTTCCCTATT	GACATTTATGGCTCCCACGT	50.0	50.0	57.9	58.0	1448 bp	0.5	59.2
ndhI_p3	CATCGAGGAGCCGTATGAAG	CATGACCTTACTTACCGGGC	55.0	55.0	57.9	58.1	1554 bp	0.5	59.1
ndhI_p4	ACCCTACAAACATGCCAAGG	GACATTTATGGCTCCCACGT	50.0	50.0	58.1	58.0	1861 bp	0.5	59.1
ndhI_p5	CCCTACAAACATGCCAAGGT	GACATTTATGGCTCCCACGT	50.0	50.0	58.1	58.0	1860 bp	0.5	59.1