Hotspot

ndhG

SSC · 123635-124165 · 531 bp

Back to Sarcandra

Pi 0.43

MarkerSeek score 77.30

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 177
pi: 0.43
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	AACTGCGTCGAGTATTGTCC	ACAATAGCTGGCCCAATAGC	50.0	50.0	58.0	58.0	1591 bp	0.5	59.8
ndhG_p2	CTGCGTCGAGTATTGTCCAA	ACAATAGCTGGCCCAATAGC	50.0	50.0	58.0	58.0	1589 bp	0.5	59.8
ndhG_p3	AACTGCGTCGAGTATTGTCC	CCAACTCCCCATCAATCTCG	50.0	55.0	58.0	58.0	1346 bp	0.5	59.6
ndhG_p4	CTGCGTCGAGTATTGTCCAA	CCAACTCCCCATCAATCTCG	50.0	55.0	58.0	58.0	1344 bp	0.5	59.6
ndhG_p5	GCCCGGTAAGTAAGGTCATG	ACAATAGCTGGCCCAATAGC	55.0	50.0	58.1	58.0	1086 bp	0.5	59.5