Hotspot

ndhF

SSC · 130448-132670 · 2223 bp

Back to Sarcandra

Pi 0.476

MarkerSeek score 80.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2223
variable_sites: 929
pi: 0.476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CAAGCTCGAGGATAGAGTGC	TTGCACACATGTCCAAGTGA	55.0	45.0	57.9	58.0	2983 bp	0.5	58.5
ndhF_p2	TCAAGCCCTGTTTTTGGTGT	TTGCACACATGTCCAAGTGA	45.0	45.0	58.1	58.0	2694 bp	0.5	58.5
ndhF_p3	CAAGCTCGAGGATAGAGTGC	GTGCACGACAAAACCTGAAC	55.0	50.0	57.9	58.2	2885 bp	0.5	57.5
ndhF_p4	TCAAGCCCTGTTTTTGGTGT	GTGCACGACAAAACCTGAAC	45.0	50.0	58.1	58.2	2596 bp	0.5	57.5
ndhF_p5	CAAGCTCGAGGATAGAGTGC	ACCTGAACCTGAAGACCTGT	55.0	50.0	57.9	58.2	2873 bp	0.5	57.4