Hotspot

ndhE

SSC · 124425-124727 · 303 bp

Back to Sarcandra

Pi 0.488

MarkerSeek score 76.80

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 303
variable_sites: 137
pi: 0.488
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	ACGTGGGAGCCATAAATGTC	CTGCGAGTTGTCTCATTCGA	50.0	50.0	58.0	58.0	1367 bp	0.5	59.6
ndhE_p2	CGAGATTGATGGGGAGTTGG	CTGCGAGTTGTCTCATTCGA	55.0	50.0	58.0	58.0	799 bp	0.5	59.5
ndhE_p3	ACGTGGGAGCCATAAATGTC	CAGTCCTCTGTTCTTGGAGC	50.0	55.0	58.0	57.9	1283 bp	0.5	59.0
ndhE_p4	ACGTGGGAGCCATAAATGTC	GTCCTCTGTTCTTGGAGCAG	50.0	55.0	58.0	57.9	1281 bp	0.5	59.0
ndhE_p5	CGAGATTGATGGGGAGTTGG	GTCCTCTGTTCTTGGAGCAG	55.0	55.0	58.0	57.9	713 bp	0.5	58.9