Hotspot

ndhA

SSC · 120485-122665 · 2181 bp

Back to Sarcandra

Pi 0.477

MarkerSeek score 79.50

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2181
variable_sites: 889
pi: 0.477
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ATGGAGATGGAAAATCCGCC	TTGGACAATACTCGACGCAG	50.0	50.0	58.0	58.0	2747 bp	0.5	59.8
ndhA_p2	CAGGCTCTGGAAGGAATTCC	TTGGACAATACTCGACGCAG	55.0	50.0	58.0	58.0	2965 bp	0.5	59.5
ndhA_p3	ATGGAGATGGAAAATCCGCC	TGCAGCTCGTACTGTTTGTT	50.0	45.0	58.0	58.1	2458 bp	0.5	59.5
ndhA_p4	ATGGAGATGGAAAATCCGCC	TTGCAGCTCGTACTGTTTGT	50.0	45.0	58.0	58.1	2459 bp	0.5	59.5
ndhA_p5	ATGGAGATGGAAAATCCGCC	AACCCAGTCACCATAGGGAA	50.0	50.0	58.0	57.9	2424 bp	0.5	59.5