Hotspot

trnL-UAG-rpl32

SSC · 128246-129181 · 936 bp

Back to Sarcandra

Pi 0.459

MarkerSeek score 80.60

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 936
variable_sites: 356
pi: 0.459
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	AGCGTGTCTACCGATTTCAC	GCACTCTATCCTCGAGCTTG	50.0	55.0	58.0	57.9	1632 bp	0.5	58.9
trnL-UAG-rpl32_p2	CAGCGTGTCTACCGATTTCA	GCACTCTATCCTCGAGCTTG	50.0	55.0	58.0	57.9	1633 bp	0.5	58.9
trnL-UAG-rpl32_p3	AAAATAGTACTTGGCGCGGT	GCACTCTATCCTCGAGCTTG	45.0	55.0	57.9	57.9	2101 bp	0.5	58.1
trnL-UAG-rpl32_p4	AAATAGTACTTGGCGCGGTT	GCACTCTATCCTCGAGCTTG	45.0	55.0	57.9	57.9	2100 bp	0.5	58.1
trnL-UAG-rpl32_p5	TCCTAAGAGCAGCGTGTCTA	GCACTCTATCCTCGAGCTTG	50.0	55.0	57.9	57.9	1642 bp	0.5	57.9