Hotspot

psaC-ndhD

SSC · 125217-125343 · 127 bp

Back to Sarcandra

Pi 0.459

MarkerSeek score 76.30

Primer available yes

Label psaC-ndhD

Feature Metadata

label_name: psaC-ndhD
feature_type: igs
region: SSC
length_bp: 127
variable_sites: 56
pi: 0.459
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhD_p1	GCTATTGGGCCAGCTATTGT	TTCCAATGCCACAGGATACG	50.0	50.0	58.0	58.0	1330 bp	0.5	59.6
psaC-ndhD_p2	TCGAATGAGACAACTCGCAG	TTCCAATGCCACAGGATACG	50.0	50.0	58.0	58.0	796 bp	0.5	59.6
psaC-ndhD_p3	GCTATTGGGCCAGCTATTGT	GTAGTGATGAAGCCCGTCAG	50.0	55.0	58.0	58.1	993 bp	0.5	59.3
psaC-ndhD_p4	TCGAATGAGACAACTCGCAG	GTAGTGATGAAGCCCGTCAG	50.0	55.0	58.0	58.1	459 bp	0.5	59.3
psaC-ndhD_p5	GCTATTGGGCCAGCTATTGT	CCCCCTGCAGTGTACAAAAT	50.0	50.0	58.0	58.1	1221 bp	0.5	59.3