Hotspot

psbT-psbN

LSC · 78062-78127 · 66 bp

Back to Sarcandra

Pi 0.121

MarkerSeek score 87.50

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 66
variable_sites: 8
pi: 0.121
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 87.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TTTTCGAGAACCACCCAAGG	TCGCCATCTCCATATCTGGT	50.0	50.0	58.0	58.0	222 bp	1.0	82.6
psbT-psbN_p2	TTTTCGAGAACCACCCAAGG	CCCTAGTCGCCATCTCCATA	50.0	55.0	58.0	58.1	228 bp	1.0	82.0
psbT-psbN_p3	TTTTCGAGAACCACCCAAGG	GATCCATTCGAGGAACACGG	50.0	55.0	58.0	58.4	136 bp	1.0	81.1
psbT-psbN_p4	TTTTCGAGAACCACCCAAGG	GGGTACGCCTTATATACCGC	50.0	55.0	58.0	57.7	184 bp	1.0	81.0
psbT-psbN_p5	TTTTCGAGAACCACCCAAGG	GTCGCCATCTCCATATCTGG	50.0	55.0	58.0	57.6	223 bp	1.0	79.7