Hotspot

trnN-GUU-trnR-ACG

IRa · 133436-134019 · 584 bp

Back to Saracha

Pi 0.00862

MarkerSeek score 64.00

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 584
variable_sites: 5
pi: 0.00862
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	673-677 bp	1.0	78.1
trnN-GUU-trnR-ACG_p2	TGGGAACGGGAATTGAACTC	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	758-762 bp	1.0	77.7
trnN-GUU-trnR-ACG_p3	TGGTCGTAGGTTCGAATCCT	GAACAGATCCAGTGGAGACG	50.0	55.0	58.2	57.7	710-714 bp	1.0	77.5
trnN-GUU-trnR-ACG_p4	CGATTGGTCGTAGGTTCGAA	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.7	57.8	677-681 bp	1.0	77.2
trnN-GUU-trnR-ACG_p5	TGGGAACGGGAATTGAACTC	GAACAGATCCAGTGGAGACG	50.0	55.0	57.8	57.7	795-799 bp	1.0	77.1