Hotspot

rps19-rpl23

IRb · 88091-89670 · 1580 bp

Back to Saracha

Pi 0.1

MarkerSeek score 97.50

Primer available yes

Label rps19-rpl23

Feature Metadata

label_name: rps19-rpl23
feature_type: igs
region: IRb
length_bp: 1580
variable_sites: 149
pi: 0.1
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 97.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl23_p1	GCCCGGGACCAAGTTACTAT	CGCTTCAACCGGGTTATTCT	55.0	50.0	59.2	58.3	1740 bp	1.0	79.4
rps19-rpl23_p2	GCCCGGGACCAAGTTACTAT	ATCATTACGCTTCAACCGGG	55.0	50.0	59.2	58.3	1747 bp	1.0	78.8
rps19-rpl23_p3	CCCGGGACCAAGTTACTATT	CGCTTCAACCGGGTTATTCT	50.0	50.0	56.6	58.3	1739 bp	1.0	77.6
rps19-rpl23_p4	GCCCGGGACCAAGTTACTAT	ACGCTTCAACCGGGTTATTC	55.0	50.0	59.2	58.6	1741 bp	1.0	77.1
rps19-rpl23_p5	CCCGGGACCAAGTTACTATT	ATCATTACGCTTCAACCGGG	50.0	50.0	56.6	58.3	1746 bp	1.0	77.0