Hotspot

petB-petD

LSC · 80261-81161 · 901 bp

Back to Saracha

Pi 0.00333

MarkerSeek score 60.70

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: LSC
length_bp: 901
variable_sites: 3
pi: 0.00333
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	CCGCTTCTTACTGCCGTATT	TATAGATCCATCCCCCGAGG	50.0	55.0	58.1	57.2	1007 bp	1.0	72.9
petB-petD_p2	CCGCTTCTTACTGCCGTATT	TTATAGATCCATCCCCCGAGG	50.0	52.4	58.1	57.9	1008 bp	1.0	70.8
petB-petD_p3	TATTGCCGCTTCTTACTGCC	TATAGATCCATCCCCCGAGG	50.0	55.0	58.3	57.2	1012 bp	1.0	70.8
petB-petD_p4	ACACACTTTTGTATTGCCGC	TATAGATCCATCCCCCGAGG	45.0	55.0	57.6	57.2	1023 bp	1.0	70.1
petB-petD_p5	TATTGCCGCTTCTTACTGCC	TTATAGATCCATCCCCCGAGG	50.0	52.4	58.3	57.9	1013 bp	1.0	68.6