Hotspot

petA-psbJ

LSC · 66673-67743 · 1071 bp

Back to Saracha

Pi 0.00374

MarkerSeek score 61.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1071
variable_sites: 4
pi: 0.00374
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GAGAAGGTTCAATTGGCCGA	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.2	58.3	1137-1138 bp	1.0	76.4
petA-psbJ_p2	TCGAGAAGGTTCAATTGGCC	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.2	58.3	1139-1140 bp	1.0	76.4
petA-psbJ_p3	AGAAGGTTCAATTGGCCGAA	GGTTCATATTCCGGGTTGGG	45.0	55.0	57.7	58.3	1136-1137 bp	1.0	75.6
petA-psbJ_p4	GAGAAGGTTCAATTGGCCGA	CATATTCCGGGTTGGGTTCA	50.0	50.0	58.2	57.6	1133-1134 bp	1.0	75.5
petA-psbJ_p5	GAGAAGGTTCAATTGGCCGA	TGGTTCATATTCCGGGTTGG	50.0	50.0	58.2	57.6	1138-1139 bp	1.0	75.5