Hotspot

psbK-psbI

LSC · 8738-9055 · 318 bp

Back to Saracha

Pi 0.00629

MarkerSeek score 56.50

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 318
variable_sites: 2
pi: 0.00629
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	ACGAAGAGTTTGAGAGTAAGCA	50.0	40.9	57.9	57.4	418-419 bp	1.0	59.4
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	ACGAAGAGTTTGAGAGTAAGCA	45.0	40.9	58.3	57.4	415-416 bp	1.0	57.4
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	ACGAAGAGTTTGAGAGTAAGCA	45.0	40.9	58.3	57.4	416-417 bp	1.0	57.4
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	ACGAAGAGTTTGAGAGTAAGCA	45.0	40.9	58.3	57.4	417-418 bp	1.0	57.4
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	AACGAAGAGTTTGAGAGTAAGCA	50.0	39.1	57.9	58.1	419-420 bp	1.0	55.7