Hotspot

rpl16

LSC · 84936-86451 · 1516 bp

Back to Saraca

Pi 0.00468
MarkerSeek score 62.70
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1516
variable_sites
7
pi
0.00468
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TCTTTTTCTTTGCTTTGGTTCGA ACGAAGACTAAGAAGCTTTTCCT 34.8 39.1 57.7 57.8 1655-1658 bp 1.0 41.3
rpl16_p2 TCTTTGCTTTGGTTCGAATTC ACGAAGACTAAGAAGCTTTTCCT 38.1 39.1 55.3 57.8 1649-1652 bp 1.0 41.3
rpl16_p3 CTTTTTCTTTGCTTTGGTTCGA ACGAAGACTAAGAAGCTTTTCCT 36.4 39.1 56.3 57.8 1654-1657 bp 1.0 41.3
rpl16_p4 TCTTTTTCTTTGCTTTGGTTCG ACGAAGACTAAGAAGCTTTTCCT 36.4 39.1 56.3 57.8 1655-1658 bp 1.0 41.3
rpl16_p5 TCTTTTTCTTTGCTTTGGTTCGA ACGAAGACTAAGAAGCTTTTCC 34.8 40.9 57.7 56.4 1655-1658 bp 1.0 41.3