Hotspot

trnN-GUU-trnR-ACG

IRa · 134688-135372 · 685 bp

Back to Saraca

Pi 0.0073
MarkerSeek score 62.90
Primer available yes
Label trnN-GUU-trnR-ACG

Feature Metadata

label_name
trnN-GUU-trnR-ACG
feature_type
igs
region
IRa
length_bp
685
variable_sites
5
pi
0.0073
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-trnR-ACG_p1 TGGGTTGAGAACGGGAATTG CAGAGGATTAGAGCACGTGG 50.0 55.0 58.1 57.8 867-887 bp 1.0 78.0
trnN-GUU-trnR-ACG_p2 GCTGTTAACTGACTGGTCGT CAGAGGATTAGAGCACGTGG 50.0 55.0 57.8 57.8 790-810 bp 1.0 77.6
trnN-GUU-trnR-ACG_p3 TGGTCGTAGGTTCGAATCCT CAGAGGATTAGAGCACGTGG 50.0 55.0 58.2 57.8 777-797 bp 1.0 77.5
trnN-GUU-trnR-ACG_p4 TGGGTTGAGAACGGGAATTG GAACAGATCCAGTGGAGACG 50.0 55.0 58.1 57.7 904-924 bp 1.0 77.4
trnN-GUU-trnR-ACG_p5 GGGTTGAGAACGGGAATTGA CAGAGGATTAGAGCACGTGG 50.0 55.0 57.8 57.8 866-886 bp 1.0 77.2