Hotspot

rpl32-trnL-UAG

SSC · 117488-118790 · 1303 bp

Back to Saraca

Pi 0.00397
MarkerSeek score 64.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1303
variable_sites
5
pi
0.00397
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCCACATCTCTTTCTACAGGG AGCGTGTCTACCGATTTCAC 52.4 50.0 58.1 58.0 1354-1398 bp 1.0 74.1
rpl32-trnL-UAG_p2 GCCACATCTCTTTCTACAGGG CAGCGTGTCTACCGATTTCA 52.4 50.0 58.1 58.0 1355-1399 bp 1.0 74.1
rpl32-trnL-UAG_p3 GCCACATCTCTTTCTACAGGG TCCTAAGAGCAGCGTGTCTA 52.4 50.0 58.1 57.9 1364-1408 bp 1.0 73.1
rpl32-trnL-UAG_p4 GCCACATCTCTTTCTACAGGG CCTAAGAGCAGCGTGTCTAC 52.4 55.0 58.1 57.8 1363-1407 bp 1.0 72.6
rpl32-trnL-UAG_p5 GCCACATCTCTTTCTACAGGG CTAAGAGCAGCGTGTCTACC 52.4 55.0 58.1 57.8 1362-1406 bp 1.0 72.6