Hotspot

trnR-ACG-trnN-GUU

IRb · 112333-113017 · 685 bp

Back to Saraca

Pi 0.0073
MarkerSeek score 62.90
Primer available yes
Label trnR-ACG-trnN-GUU

Feature Metadata

label_name
trnR-ACG-trnN-GUU
feature_type
igs
region
IRb
length_bp
685
variable_sites
5
pi
0.0073
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-trnN-GUU_p1 CAGAGGATTAGAGCACGTGG TGGGTTGAGAACGGGAATTG 55.0 50.0 57.8 58.1 867-887 bp 1.0 78.0
trnR-ACG-trnN-GUU_p2 CAGAGGATTAGAGCACGTGG GCTGTTAACTGACTGGTCGT 55.0 50.0 57.8 57.8 790-810 bp 1.0 77.6
trnR-ACG-trnN-GUU_p3 CAGAGGATTAGAGCACGTGG TGGTCGTAGGTTCGAATCCT 55.0 50.0 57.8 58.2 777-797 bp 1.0 77.5
trnR-ACG-trnN-GUU_p4 GAACAGATCCAGTGGAGACG TGGGTTGAGAACGGGAATTG 55.0 50.0 57.7 58.1 904-924 bp 1.0 77.4
trnR-ACG-trnN-GUU_p5 CAGAGGATTAGAGCACGTGG GGGTTGAGAACGGGAATTGA 55.0 50.0 57.8 57.8 866-886 bp 1.0 77.2