Hotspot

trnT-UGU-trnL-UAA

LSC · 49348-50326 · 979 bp

Back to Saraca

Pi 0.0171
MarkerSeek score 71.60
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
979
variable_sites
15
pi
0.0171
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 55.0 50.0 57.9 58.1 982-1037 bp 1.0 83.6
trnT-UGU-trnL-UAA_p2 GGAATCGAACCGATGACCAT TAGCGTCTACCAATTTCGCC 50.0 50.0 57.8 58.1 1014-1069 bp 1.0 82.7
trnT-UGU-trnL-UAA_p3 TGGAAAAGAAAAGCCGGCTA TAGCGTCTACCAATTTCGCC 45.0 50.0 57.7 58.1 1036-1091 bp 1.0 82.3
trnT-UGU-trnL-UAA_p4 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 55.0 50.0 57.9 58.2 988-1043 bp 1.0 82.2
trnT-UGU-trnL-UAA_p5 GGAATCGAACCGATGACCAT AGTCCGTAGCGTCTACCAAT 50.0 50.0 57.8 58.2 1020-1075 bp 1.0 81.3