Hotspot

psbZ-trnG-GCC

LSC · 38842-39454 · 613 bp

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Pi 0.00559
MarkerSeek score 64.60
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
613
variable_sites
3
pi
0.00559
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.88
markerseek_score
64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CTTCTCCTGATGGTTGGTCG CGTCTTCTCCTTGGCAAAGA 55.0 50.0 58.0 57.8 692-767 bp 1.0 82.8
psbZ-trnG-GCC_p2 TTGCTTCTCCTGATGGTTGG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.8 57.8 695-770 bp 1.0 81.3
psbZ-trnG-GCC_p3 TCCTGATGGTTGGTCGAGTA CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.8 57.8 688-763 bp 1.0 81.2
psbZ-trnG-GCC_p4 CTTCTCCTGATGGTTGGTCG GGATAGCGGGAATTGAACCC 55.0 55.0 58.0 58.4 713-788 bp 1.0 81.0
psbZ-trnG-GCC_p5 GCTTCTCCTGATGGTTGGTC CGTCTTCTCCTTGGCAAAGA 55.0 50.0 58.3 57.8 693-768 bp 1.0 80.9