Hotspot

trnS-UGA-psbZ

LSC · 38268-38652 · 385 bp

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Pi 0.0131
MarkerSeek score 62.80
Primer available yes
Label trnS-UGA-psbZ

Feature Metadata

label_name
trnS-UGA-psbZ
feature_type
igs
region
LSC
length_bp
385
variable_sites
5
pi
0.0131
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-UGA-psbZ_p1 GAGCTATCAACCACTCAGCC CGACCAACCATCAGGAGAAG 55.0 55.0 58.1 58.0 511-515 bp 1.0 80.9
trnS-UGA-psbZ_p2 GGAGCTATCAACCACTCAGC CGACCAACCATCAGGAGAAG 55.0 55.0 58.1 58.0 512-516 bp 1.0 80.8
trnS-UGA-psbZ_p3 CAAGACCGGAGCTATCAACC CGACCAACCATCAGGAGAAG 55.0 55.0 58.1 58.0 519-523 bp 1.0 80.8
trnS-UGA-psbZ_p4 TTCGTCGAGCAAAAGGAGAG CGACCAACCATCAGGAGAAG 50.0 55.0 57.9 58.0 589-593 bp 1.0 80.6
trnS-UGA-psbZ_p5 ACAAACTCATTCGTCGAGCA CGACCAACCATCAGGAGAAG 45.0 55.0 57.9 58.0 598-602 bp 1.0 79.9