Hotspot

atpH-atpI

LSC · 14176-15381 · 1206 bp

Back to Saraca

Pi 0.0372
MarkerSeek score 83.90
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1206
variable_sites
43
pi
0.0372
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 AATAGAAGCAAGCCCGACAG TTTGCAACTTTAGCTGCTGC 50.0 45.0 58.0 57.9 1326-1391 bp 1.0 84.1
atpH-atpI_p2 ATAACGGAAGCGGCAGAAAT TTTGCAACTTTAGCTGCTGC 45.0 45.0 58.0 57.9 1292-1357 bp 1.0 84.1
atpH-atpI_p3 TGGTCCAATAGAAGCAAGCC TTTGCAACTTTAGCTGCTGC 50.0 45.0 57.9 57.9 1332-1397 bp 1.0 83.3
atpH-atpI_p4 ATAACGGAAGCGGCAGAAAT TCCCTGTCATGTTCCTTGGA 45.0 50.0 58.0 58.3 1342-1407 bp 1.0 83.0
atpH-atpI_p5 AATAGAAGCAAGCCCGACAG TCCCTGTCATGTTCCTTGGA 50.0 50.0 58.0 58.3 1376-1441 bp 1.0 82.9