Hotspot

trnK-UUU-rps16

LSC · 4495-5406 · 912 bp

Back to Saraca

Pi 0.00877
MarkerSeek score 56.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
912
variable_sites
8
pi
0.00877
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGCTGGTATGAACGAATCCA CAAAAAGGGCGGGTGTTTTT 50.0 45.0 58.1 58.0 1064-1072 bp 1.0 79.8
trnK-UUU-rps16_p2 TCGCTGGTATGAACGAATCC CAAAAAGGGCGGGTGTTTTT 50.0 45.0 57.8 58.0 1065-1073 bp 1.0 78.6
trnK-UUU-rps16_p3 CGCTGGTATGAACGAATCCA GGCGGGTGTTTTTATCGAAC 50.0 50.0 58.1 57.7 1057-1065 bp 1.0 78.1
trnK-UUU-rps16_p4 TCGCTGGTATGAACGAATCC GGCGGGTGTTTTTATCGAAC 50.0 50.0 57.8 57.7 1058-1066 bp 1.0 76.8
trnK-UUU-rps16_p5 AGTCGCACTTAAAAGCCGAG CAAAAAGGGCGGGTGTTTTT 50.0 45.0 58.6 58.0 1016-1024 bp 1.0 75.8