Hotspot

trnH-GUG-psbA

LSC · 83-508 · 426 bp

Back to Saraca

Pi 0.0415
MarkerSeek score 75.30
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
426
variable_sites
16
pi
0.0415
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGCCAT 55.0 55.0 58.1 58.0 463-493 bp 1.0 85.6
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA CCTCTAGACCTAGCTGCCAT 50.0 55.0 57.9 58.0 477-507 bp 1.0 85.5
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC CCTCTAGACCTAGCTGCCAT 50.0 55.0 57.9 58.0 476-506 bp 1.0 85.5
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC CCTCTAGACCTAGCTGCCAT 50.0 55.0 57.9 58.0 478-508 bp 1.0 85.5
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 466-496 bp 1.0 85.0