Hotspot

clpP

LSC · 72378-74513 · 2136 bp

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Pi 0.000939

MarkerSeek score 58.60

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2136
variable_sites: 3
pi: 0.000939
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CATTTGCGCGACATCACTG	ACGTTTCCACATCAAAGTGA	52.6	40.0	58.1	55.5	2202-2212 bp	1.0	52.0
clpP_p2	CGCGACATCACTGCTATTTT	ACGTTTCCACATCAAAGTGA	45.0	40.0	56.9	55.5	2196-2206 bp	1.0	51.9
clpP_p3	CATTTGCGCGACATCACTG	ACGTTTCCACATCAAAGTGAA	52.6	38.1	58.1	56.3	2202-2212 bp	1.0	50.3
clpP_p4	CGCGACATCACTGCTATTTT	ACGTTTCCACATCAAAGTGAA	45.0	38.1	56.9	56.3	2196-2206 bp	1.0	50.2
clpP_p5	GCGCGACATCACTGCTATTT	ACGTTTCCACATCAAAGTGA	50.0	40.0	59.3	55.5	2197-2207 bp	1.0	49.9