Hotspot

atpA

LSC · 10489-12012 · 1524 bp

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Pi 0.00175

MarkerSeek score 59.80

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 4
pi: 0.00175
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	AAAGATTAAGAGTGATCGAATTT	TGACACGTTTGAGGTGATGG	26.1	50.0	52.2	58.1	1646 bp	1.0	40.2
atpA_p2	AAGATTAAGAGTGATCGAATTTT	TGACACGTTTGAGGTGATGG	26.1	50.0	52.2	58.1	1645 bp	1.0	40.2
atpA_p3	AGATTAAGAGTGATCGAATTTTT	TGACACGTTTGAGGTGATGG	26.1	50.0	52.2	58.1	1644 bp	1.0	40.2
atpA_p4	AAGATTAAGAGTGATCGAATTTT	GACACGTTTGAGGTGATGGA	26.1	50.0	52.2	57.8	1644 bp	1.0	40.2
atpA_p5	AGATTAAGAGTGATCGAATTTTT	GACACGTTTGAGGTGATGGA	26.1	50.0	52.2	57.8	1643 bp	1.0	40.2